Culture of Nitella and chara


Composition of Culture Medium

1 Major Ingredients

Compounds Formula Final Concentration
Calcium Chloride dihydrate, CaCl2?H2O 300 mM
Potassium Chloride KCl 60 mM
Sodium Nitrate NaNO3 120 mM
Sodium Dihydrogen Phosphate NaH2PO4 H2O 40 mM
Magnesium Sulfate heptahydrate MgSO47H20 150 mM
Compounds Formula Final Concentration
MnEDTA   10 mM(0.3mM*)
ZnEDTA   2 mM

Nitella expansa: Mn 3 mM Zn 1mM , Chara australis: Mn 10mM , Zn 3 mM

Minor Ingredients B (Minor Elements)

Compounds Formula Final Concentration
Boric Acid H3BO3 10 mM
CuEDTA 3H20   0.03 mM
Potassium Iodide   0.05 mM
Sodium Molybdate dihydrate Na2MoO42H20 0.01mM
Cobalt Chloride hexahydrate CoCl26H20 0.001 mM

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