Experimental Protocols and Materials Revised on 6/1, 1998, 10:20


Morphological observations

  1. Fluorescence microscopy
  2. Electron microscopy

Cells

  1. grow plants
  2. tissue culture
  3. Isolation of giant protoplasts
  4. handling of cells

Protein related methods

  1. Isolation and purification of proteins
  2. High-speed SDS-PAGE
  3. Western blotting
  4. Preparation of antibodies

Cytoskeleton related methods

  1. Isolation of the cytoskeleton fractions from plant cells
    Methods in Cell Biology 50B Home Page Chapter 16.
  2. Cell Images

 

Gene related methods

  1. Isolation of DNA (genomic, plasmid, phage etc)
  2. Isolation of RNA from plant materials
  3. Construction of Genomic library
  4. Construction of cDNA library
  5. Isolation of plant nuclei
  6. Isolation and characterization of polysomes from plant cells
    Methods in Cell Biology 50B Home Page Chapter 15.

Biophysics related

  1. Insertion of electrodes into protoplasts

Miscellaneous

  1. Gradient centrifugation
  2. Preparing sucrose gradients
  3. Culture Media for Chara and Nitella

Equipments and Facillities


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