Culture of Nitella and chara
Composition of Culture Medium
1 Major Ingredients
| Compounds | Formula | Final Concentration |
| Calcium Chloride dihydrate, | CaCl2?H2O | 300 mM |
| Potassium Chloride | KCl | 60 mM |
| Sodium Nitrate | NaNO3 | 120 mM |
| Sodium Dihydrogen Phosphate | NaH2PO4· H2O | 40 mM |
| Magnesium Sulfate heptahydrate | MgSO4·7H20 | 150 mM |
| Compounds | Formula | Final Concentration |
| MnEDTA | 10 mM(0.3mM*) | |
| ZnEDTA | 2 mM |
Nitella expansa: Mn 3 mM Zn 1mM , Chara australis: Mn 10mM , Zn 3 mM
Minor Ingredients B (Minor Elements)
| Compounds | Formula | Final Concentration |
| Boric Acid | H3BO3 | 10 mM |
| CuEDTA 3H20 | 0.03 mM | |
| Potassium Iodide | 0.05 mM | |
| Sodium Molybdate dihydrate | Na2MoO42H20 | 0.01mM |
| Cobalt Chloride hexahydrate | CoCl26H20 | 0.001 mM |